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A large autoclave with its door open. Photo by Carol M. Highsmith/LOC

Autoclaves

Be glad, be very glad that your eyes aren't as powerful as electron microscopes. If they were, you'd see the world around you crawling with all kinds of horrible bugs. How filthy and nasty life would seem! Just as well, then, that we have autoclaves: machines for sterilizing things and keeping them germ-free. They're a bit like giant pressure cookers that use the power of steam to kill off microbes that might survive a simple wash or wipe with hot water and detergents. They're easy to use, good for wholesale sterilizing (large quantities of equipment), and because they use steam, are relatively economical to operate. Let's take a closer look at what they are and how they work!

Photo: Looking inside the open door of a large autoclave. Note the gasket seal on the door to keep the steam inside and the pressure gauges on top. Photo by Carol M. Highsmith courtesy of The George F. Landegger Collection of Alabama Photographs in Carol M. Highsmith's America, Library of Congress, Prints and Photographs Division.

Contents

  1. What does an autoclave do?
  2. Why is pressure important in an autoclave?
  3. How does an autoclave work?
  4. How do you use an autoclave?
  5. Industrial and scientific autoclaves
  6. Who invented autoclaves?
  7. What's the difference between an autoclave and a pressure cooker?
  8. Find out more

What does an autoclave do?

Although autoclaves have many important scientific and industrial uses, which we'll cover later, the main focus of this article is going to be on how these handy machines are used in sterilization.

Green-suited medics test a microprocessor-controlled Hanshin medical HS-4086G steam autoclave

Photo: Testing a steam-sterilizing autoclave before use. This one is a microprocessor-controlled Hanshin HS-4085G, which can sterilize loads of up to 85.6l (22.6 gal) at temperatures up to 135°C (275°F). Photo by Roadell Hickman courtesy of US Navy.

You've probably heard of pressure cookers? They were all the rage until microwave ovens became popular in the 1980s. They're like over-sized saucepans with lids that seal on tightly and, when you fill them with water, they produce lots of high-pressure steam that cooks your food more quickly (if you want to know more, please see the box at the bottom of this page). Autoclaves work in a similar way, but they're typically used in a more extreme form of cooking: to blast the bugs and germs on things with steam long enough to sterilize them. The extra pressure in an autoclave means that water boils at a temperature higher than its normal boiling point—roughly 20°C hotter—so it holds and carries more heat and kills microbes more effectively. A lengthy blast of high-pressure steam is much more effective at penetrating and sterilizing things than a quick wash or wipe in ordinary hot water and disinfectant. According to one recent review by scientists from New Zealand: "Steam sterilization (autoclaving) is the most widely used method for sterilization and is considered the most robust and cost-effective method for sterilization of medical devices."

Why is pressure important in an autoclave?

Inflating a bicycle tire: the atoms in the gas get closer together as you compress them.

Artwork: Tires have springiness because of the energetic air molecules inside them, which causes pressure.

Pressure is the way a force acts over a surface. If you pump air into a bicycle tire, the energetic molecules of gas rush about inside, colliding with the tire walls and pressing outward. The tire stays springy and inflated because the air molecules push its inner walls with as much (or greater) force than the air molecules outside are pushing the outer walls. If you heat up a tire, you give the air molecules more energy. They rush about faster, collide with the rubber walls of the tire more often and exert even more force. The tire feels more pumped up or, if you're unlucky, bursts!

In physics, we say the pressure on a surface is the force pressing on it divided by the area over which the force acts:

Pressure = Force / Area

This simple equation tells you that if you apply a given force to half the area, you double the pressure. Apply the force to twice the area and you halve the pressure.

Pushing a thumbtack into a wall.

Photo: Thumbtacks (drawing pins) use the science of pressure. The difference in area between the head you push and the sharp point that enters the wall effectively magnifies your pushing force.

It's very helpful to know about pressure in everyday life. Suppose you want to put a poster up on your bedroom wall. Assuming you don't have a hammer, you'll find it much easier to use thumbtacks (drawing pins) than nails. A thumbtack has a huge flat head connected to a very thin pin with a sharp tip. When you push on the flat head, you apply a certain amount of force to a fairly big area. The force is transmitted right through the pin to the tip, where it now acts on an area of metal that's maybe 100 times smaller. So the pressure on the tip is effectively 100 times greater—and that's why the pin enters your wall so easily. Snowshoes and tractor tires use exactly the same principle only in reverse. They spread weight (the force of gravity) over a bigger area to stop your body (or a machine) from sinking into soft ground.

How pressure and temperature affect boiling

Suppose you have a saucepan full of potatoes that you want to cook. You fill the pan with water, put it on a hot stove, and wait for the water to boil. Now you probably think the water will boil "when it's hot enough"—and that's true, but only half true. The water will actually boil when most of the molecules it contains have enough energy to escape from the liquid and form water vapor (steam) above it. The hotter the water is, the more energetic the molecules are and the more easily they can escape. So temperature plays an important part in making things boil.

But pressure is important too. The higher the pressure of the air above the water, the harder it is for the molecules to break free; the lower the pressure, the easier it is. If you've ever tried making a cup of tea on a mountain with a portable camping stove, you'll know the water boils at a lower temperature at high altitudes. That's because air pressure falls the higher up you go. At the top of Mount Everest, air pressure is about a third of what it would be at sea level, so water boils at roughly 70°C or 158°F (see why on this MadSci forum posting). Mountain-top tea tastes pretty disgusting because the water boils at too low a temperature: even though it's boiling, the water is too cold to "cook" the tea leaves properly.

Read more about pressure, temperature, and how molecules behave when liquids boil.

How does an autoclave work?

An autoclave is essentially just a large steel vessel through which steam or another gas is circulated to sterilize things, perform scientific experiments, or carry out industrial processes. Typically the chambers in autoclaves are cylindrical, because cylinders are better able to withstand extreme pressures than boxes, whose edges become points of weakness that can break. The high-pressure makes them self-sealing (the words "auto" and "clave" mean automatic locking), though for safety reasons most are also sealed manually from outside. Just like on a pressure cooker, a safety valve ensures that the steam pressure cannot build up to a dangerous level.

Photo: A typical medical autoclave.

Photo: Closing the door on a typical laboratory autoclave. Note the large handle on the right being used to seal the door completely. Also note the dials on the right-hand side that indicate temperature and pressure. Photo by PHAA Sarna courtesy of US Navy.

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How do you use an autoclave?

Once the chamber is sealed, all the air is removed from it either by a simple vacuum pump (in a design called pre-vacuum) or by pumping in steam to force the air out of the way (an alternative design called gravity displacement). Next, steam is pumped through the chamber at a higher pressure than normal atmospheric pressure so it reaches a temperature of about 121–140°C (250–284°F). Once the required temperature is reached, a thermostat kicks in and starts a timer. The steam pumping continues for a minimum of about 3 minutes and a maximum of about 15–20 minutes (higher temperatures mean shorter times)—generally long enough to kill most microorganisms. The exact sterilizing time depends on a variety of factors, including the likely contamination level of the items being autoclaved (dirty items known to be contaminated will take longer to sterilize because they contain more microbes) and how the autoclave is loaded up (if steam can circulate more freely, autoclaving will be quicker and more effective).

Simplified artwork showing the components inside an autoclave.

Artwork: How an autoclave works (simplified): (1) Steam flows in through a pipe at the bottom and around a closed jacket that surrounds the main chamber (2), before entering the chamber itself (3). The steam sterilizes whatever has been placed inside (in this case, three blue drums) (4) before exiting through an exhaust pipe at the bottom (5). A tight door lock and gasket seal (6) keeps the steam securely inside. A safety valve (7) similar to the ones on a pressure cooker will pop out if the pressure gets too high.

Autoclaving is a bit like cooking, but as well as keeping an eye on the temperature and the time, the pressure matters too! Safety is all-important. Since you're using high-pressure, high-temperature steam, you have to be especially careful when you open an autoclave that there is no sudden release of pressure that could cause a dangerous steam explosion.

Two engineers load or unload an autoclave with a piece of aluminum.

Photo: Scientific autoclaving: US Navy engineers load an autoclave with a piece of aluminum to heat and bond a composite patch onto it. Photo by Jonathan L. Correa courtesy of US Navy.

Industrial and scientific autoclaves

Although best known as sterilizers, autoclaves can also be used to carry out all sorts of industrial processes and scientific experiments that work best at high-temperatures and pressures.

Unlike sterilizing autoclaves, which usually circulate steam, industrial and scientific autoclaves may circulate other gases to encourage particular chemical reactions to take place. Industrial autoclaves are often used for "curing" materials (applying heat to encourage the formation of long-chain polymer molecules).

Artwork: A simple industrial autoclave invented by Oliver Sleeper and patented in 1922.

Artwork: A simple industrial autoclave from the early 20th century, designed for manufacturing various industrial chemicals using acids. It's essentially a reinforced, acid-resistant cooking vessel (blue) with a removable screw-on top (orange). You can add chemical ingredients through the smaller screw-on entry hole (green) and stir them using a gear-driven agitator (red). This is more like a modern pressure cooker than an autoclave. From US Patent 1,426,920: Autoclave by Oliver Sleeper, August 22, 1922, courtesy of US Patent and Trademark Office.

Some examples of how industrial autoclaves are used:

Some autoclaves combine elements of both sterilization and industrial manufacture. For example, natural cork (wooden) bottle stoppers have to be boiled and sterilized before they're suitable for use. Traditionally, that was done in large water tanks; now it's much more likely to be done on a large scale in computer-controlled, industrial autoclaves.

Who invented autoclaves?

Photo: An unusual cylindrical autoclave being used to carry out scientific experiments onboard the Space Shuttle in 1995.

Photo: Scientific autoclave: Inspecting a crystal grown in microgravity inside a cylindrical autoclave. This scientific experiment was carried out onboard the Space Shuttle in October 1995. Photo by courtesy of NASA Marshall Space Flight Center (NASA-MSFC).

What's the difference between an autoclave and a pressure cooker?

Want to cook your dinner in a fraction of the time? You could use a microwave to zap it with energetic waves. But another popular solution is to seal it in a pressure cooker: a kind of saucepan that cooks things quicker by boiling them at a higher temperature than usual. Although considered old-fashioned by some, pressure cookers are still a convenient and economical way to prepare food. The basic concept—using pressure to achieve a higher temperature—is the same as what happens in an autoclave.

Pressure cooker on a stove showing steam valve.

Photo: A pressure cooker in action. Notice the valve on the top through which steam escapes and the double handle arrangement used to lock the lid. Photo by George Danor, US Office of War Administration, courtesy of US Library of Congress.

We've already seen that high pressure raises the boiling point of water. Suppose we could somehow arrange things so that the air above our saucepan was actually at a much higher pressure than usual. That would make the water boil at a significantly hotter temperature, which would make the potatoes cook more quickly.

This is the basic idea behind pressure cookers. A pressure cooker is a big steel saucepan with a tight-fitting lid. The outer edge of the lid has a thick circle of rubber called a gasket that fits between the bottom of the lid and the top of the pan to make a really tight seal.

When you fill the pan with water and place it on the stove, the water heats up and some of its molecules escape to form steam up above it. With a normal pan, the steam would just drift off into your kitchen and disappear. But with a pressure cooker, the gasket and lid stop the steam escaping so the pressure soon builds up. Although the water inside the pan boils, the higher pressure means it boils at a higher temperature than normal that cooks your food more quickly. A special valve on the top of the lid allows a small amount of steam to escape, keeping the pressure higher than normal but not so high that the cooker explodes. If the pressure inside the pan builds up too much, the valve pops right out, rapidly lowering the pressure to a safe level again.

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For deeper technical details, try this small selection of the many autoclave designs that have been patented:

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Text copyright © Chris Woodford 2008, 2020. All rights reserved. Full copyright notice and terms of use.

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Woodford, Chris. (2008/2020) Autoclaves. Retrieved from https://www.explainthatstuff.com/autoclaves.html. [Accessed (Insert date here)]

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